Buyang Huanwu Decoction promotes the neurological recovery of traumatic spinal cord injury via inhibiting apoptosis, inflammation, and oxidative stress

Background The incidence rate of spinal cord injury (SCI) is increasing, and the mortality or disability caused by SCI remains high in world. Buyang Huanwu Decoction (BYHWD) a kind Traditional Chinese medicine, it believed to be effective several kinds nervous system diseases. Whether BYHWD could improve potential function mechanism remain unclear. Methods animal model was established damaging T10 cord. Animals experiments included five groups as follows: Sham, SCI, SCI+BYHWD, SCI+mesenchymal stromal cells (MSCs), SCI+BYHWD+MSCs. H2O2-treated (100 µM, 6 h) were used simulate damage vitro, which control, H2O2, H2O2+BYHWD, H2O2+MSCs, H2O2+BYHWD+MSCs. behavioral evaluated with Tarlov inclined plated test score. Western blot analysis immunohistochemical staining detect protein expression. levels superoxide dismutase (SOD), catalase (CAT), malondiadehyde (MDA), interleukin (IL)-1β, tumor necrosis factor-α, IL-6 serum measured commercial enzyme-linked immunosorbent assay kits. terminal deoxynucleotidyl transferase dUTP nick end labeling flow cytometry performed measure apoptosis vivo vitro levels. Gene expression profiling analyze differential genes. Results suppressed accelerating cell proliferation after SCI. Recovery neurofunction, inhibition inflammatory response, oxidative condition achieved MSCs. gp130/Janus kinase/signal transducers activator transcription (JAK/STAT) MSCs, both vitro. MSCs markedly promoted viability inhibited apoptosis. Greater gene difference observed between group control H2O2 through analysis. recovery effects traumatic similar synergies items. Conclusion increase score Basso, Beatie, Bresnahan functional score, inhibit apoptosis, level gp130/JAK/STAT axis BYHWD. might new therapeutic strategy for prevention treatment